透明质酸-荧光素 荧光标记 HA-FITC | Hyaluronate Fluorescein (FITC labeled Hyaluronic Acid)

Description:

Hyaluronic Acid is labeled with fluorescein (FITC), Ex/Em wavelength 494/518 nm. Purity: >95% powder. Degree of substitution (DoS) of FITC: 1 mol % substitution unless the DoS is specifically noted in the catalog number. For example HA-809-5% means that the DoS for FITC is 5% in this particular HA-FITC product. HA labeled with dyes of different labeling ratios may be provided by custom synthesis. HA labeled with other rhodamine dyes such as Texas Red, Sulforhodamine 101 may be offered by custom synthesis.

The dye substitution or labeling ratio is the percentage of HA disaccharide monomers that are functionalized with dye groups. You may calculate the number of dye by 1) dividing the MW of HA by the MW of one disaccharide monomer to derive the number of disaccharide monomers in HA; 2) then multiple the number of disaccharide monomers by the labeling ratio to get the number of dye in each HA polymer.

References:

1. Enhanced articular cartilage by human mesenchymal stem cells in enzymatically mediated transiently RGDS-functionalized collagen-mimetic hydrogels, Acta Biomaterialia, 2017, 51, 75-88, Text.

2. Targeted delivery of hyaluronic acid to the ocular surface by a polymer-peptide conjugate system for dry eye disease, Acta Biomaterialia, Volume 55, June 2017, Pages 163-171, Text.

3. Harnessing the Versatility of Bacterial Collagen to Improve   the Chondrogenic Potential of Porous Collagen Scaffolds, Adv Healthc Mater. 2016;5(13):1656-66, Text.

4. Topical delivery of hyaluronic acid into skin using SPACE-peptide carriers, Journal of Controlled Release, 2014, 173(10), pp 67–74, Text.

5. A hyaluronic acid-binding contact lens with enhanced water retention, Contact Lens and Anterior Eye, 2014, 38(2), pp 79–84, Text.

6. Preparation and properties of fluorescein-labeled hyaluronate. Carbohydrate Research,1975,44(2):251-7.

7. Temporally degradable collagen–mimetic hydrogels tuned to chondrogenesis of human mesenchymal stem cells, Biomaterials, 99, 2016, 56-71, Text.

8. Hyaluronan modulation impacts Staphylococcus aureus biofilm infection.   Infection and Immunity (2016): IAI-01418. Text.

9. Synergistic Antitumor Activity of Camptothecin-Doxorubicin Combinations and their Conjugates with Hyaluronic Acid. J Control Release. 2015 Jul 28;210:198-207.Text.