高抗菌和高粘附力的透明质酸水凝胶用于伤口修复《Biomacromolecules》

高抗菌和高粘附力的透明质酸水凝胶用于伤口修复

摘要

伴随伤口愈合的细菌感染通常会给患者带来更严重的健康危害。因此，迫切需要探索一种既能促进伤口修复又具有抗菌能力的伤口敷料。这里，我们通过氧化还原引发的甲基丙烯酸透明质酸（HAMA）、5，10，15，20-四（4-甲基丙烯酸苯基）卟啉（TPP）和多巴胺甲基丙烯酰胺（DMA）的交联反应构建了一种多功能水凝胶敷料，命名为HAMA-TPP-DMA，具有广谱光动力学抗菌能力，其中TPP光敏剂单元的聚集可以被大大抑制以产生更多的单线态氧。该水凝胶具有优异的生物降解性和生物相容性，为伤口愈合提供了有利条件。此外，将多巴胺掺入水凝胶使伤口敷料具有增强的粘附性，有利于伤口修复。更重要的是，体外抗菌实验和体内小鼠伤口模型表明，HAMA-TPP-DMA水凝胶可以显著抵抗细菌并加速小鼠伤口愈合（15天后闭合率>98%）。因此，这种具有优异抗菌感染和伤口愈合能力的水凝胶敷料为伤口修复提供了一种有前景的策略。

Highly Antibacterial and Adhesive Hyaluronic Acid Hydrogel for Wound Repair

Baoxuan Huang, Dan Hu, Alideertu Dong, Jia Tian*, and Weian Zhang*

Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, People's Republic of China

Biomacromolecules 2022, XXXX, XXX, XXX-XXX

Publication Date: November 2, 2022

https://doi.org/10.1021/acs.biomac.2c00950

Abstract

Bacterial infections accompanied with wound healing often lead to more serious health hazards to patients. Therefore, it is urgent to explore a wound dressing that can promote wound repair while possessing antibacterial capability. Here, we constructed a multifunctional hydrogel dressing by a redox-initiated cross-linking reaction of methacrylated hyaluronic acid (HAMA), 5,10,15,20-tetra (4-methacrylate phenyl) porphyrin (TPP), and dopamine methacrylamide (DMA), named HAMA-TPP-DMA, with broad-spectrum photodynamic antibacterial capability, where the aggregation of TPP photosensitizer units could be greatly inhibited to produce more singlet oxygen. The hydrogel has excellent biodegradability and biocompatibility, providing favorable conditions for wound healing. Furthermore, the incorporation of dopamine into the hydrogel gives the wound dressing with enhanced adhesiveness, benefiting for the wound repair. More importantly, the antibacterial experiments in vitro and mice wound models in vivo showed that the HAMA-TPP-DMA hydrogel can significantly resist bacteria and accelerate the wound healing in mice (the closure rate > 98% after 15 days). Thus, this hydrogel dressing with superior antibacterial infection and wound healing capability provides a promising strategy in wound repair.

SUBJECTS:

• Bacteria,

• Hydrogels,

• Infectious diseases,

• Irradiation,

• Wound healing